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ansZ [2019-09-02 11:15:58]
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ansZ [2019-09-02 11:15:58]

L-asparaginase
Locus
BSU_02690
Isoelectric point
6.15
Molecular weight
39.94 kDa
Protein length
375 aa Sequence Blast
Gene length
1128 bp Sequence Blast
Function
utilization of asparagine
Product
L-asparaginase
Essential
no
E.C.
3.5.1.1
Synonyms
yccC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of asparagine/ aspartate

    • Gene

    Coordinates
    290,915 → 292,042

    The protein

    Catalyzed reaction/ biological activity

  • H2O + L-asparagine --> L-aspartate + NH4+(according to UniProt)

    • Protein family

  • asparaginase 1 family (with AnsA, according to UniProt)

    • Paralogous protein(s)

  • AnsA

    • Structure

  • 1ZCF (from Pectobacterium atrosepticum scri1043, 55% identity, 73% similarity)

    • Expression and Regulation

    Operons

    Genes
    ansZ
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon
  • CodY: repression, PubMed, in CodY regulon

    • Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
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    Biological materials

    Mutant

  • BKE02690 (ΔansZ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATTTCTCTCCTTACA, downstream forward: _UP4_TGAAGAAAAGAAGGCGAATA
  • BKK02690 (ΔansZ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATTTCTCTCCTTACA, downstream forward: _UP4_TGAAGAAAAGAAGGCGAATA

    • References

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